The Marine Life Information Network

Global warming (extreme)

Extreme emission scenario (by the end of this century 2081-2100) benchmark of:

• A 5°C rise in SST and NBT (coastal to the shelf seas),

• A 6°C rise in surface air temperature (in eulittoral and supralittoral habitats).

• A 1°C rise in Deep-sea habitats (>200 m) off the continental shelf, and

• A 5°C rise in surface air temperature in intertidal habitats exclusive to Scotland. Further detail.

Evidence

Intertidal species are exposed to extremes of high and low air temperatures during periods of emersion. They must also be able to cope with sharp temperature fluctuations over a short period during the tidal cycle. In winter air temperatures are colder than the sea, conversely in summer air temperatures are much warmer than the sea. Species that occur in the intertidal are, therefore, generally adapted to tolerate a range of temperatures, with the width of the thermal niche positively correlated with the height of the shore that the animal usually occurs (Davenport & Davenport, 2005). The species present in this biotope are present in the mid and/or upper shore and therefore are likely to have broad temperature tolerances and to be tolerant of temperature fluctuations.

The amphipods that occur within this habitat are mobile and can avoid unfavourable conditions to some extent. The life cycle of Bathyporeia spp. vary between locations, which is related to temperature (Mettam, 1989). Preece (1971) tested the temperature tolerance of Bathyporeia pilosa in the laboratory. Individuals acclimated to 15°C for 24 hours were exposed to temperature increases (water temperature raised by 0.2°C/minute). As test temperatures were reached individuals were removed, placed in seawater at 4°C and allowed to recover for 24 hours at which point mortalities were tested. Amphipods were also allowed to bury into sediments and held at test temperatures for 24 hours of 32.5°C, 31.8°C and 29.5°C before being allowed to recover in fresh seawater at 15°C for a further 24 hours, before mortalities were assessed. The upper lethal temperature (the temperature at which 50% of individuals died) for adult males and gravid females of Bathyporeia pilosa was 39.4°C. These tests measures short-term exposure only and species had a lower tolerance for longer-term (24-hour exposure). No mortality occurred for Bathyporeia pilosa individuals held at 29.5°C and 30.8°C. However, 15% of individuals exposed to water temperatures of 31.8°C and 96% at 32.5°C died.

The tolerance of Bathyporeia pilosa to high temperatures may be because it is found in the intertidal, although its ability to withstand long-term changes in temperature is more difficult to discern. Whilst Bathyporeia pilosa can tolerate extremely high temperatures for short periods, this species has a limited distribution from the coast of Norway down to the French coast of the Bay of Biscay (Speybroeck et al., 2008), which suggests that it may be susceptible to ocean warming. As temperatures increase, non-lethal effects of heat stress may lead to changes in population dynamics and its biogeographic range moving northwards to compensate.

Corophium volutator occurs in the North-East Atlantic (NEA) from northern Norway to the Bay of Biscay, and in the North-West Atlantic in the Bay of Fundy and along the coast of Maine (www.obis.org). Whilst it is also thought to be present in the Mediterranean Sea, the Black Sea and the Azov Sea (Neal & Avant, 2006), it is expected that these records refer to Corophium orientale, which is a closely related species originally regarded as a subspecies of Corophium volutator (Crawford, 1937). Corophium arenarium has a distribution from Scotland to the French Coast of the Bay of Biscay. When Corophium volutator was exposed to choice experiments to assess temperature preferences between 0°C and 30°C, it was shown that this species preferred temperatures in the range of 15 - 20°C, although activity peaked at 25°C and it was able to withstand temperatures of 30°C (Meadows & Ruagh, 1981).

Temperature increases may lead to indirect effects on populations of the characterizing species, through changes in the distribution and prevalence of parasites and pathogens (see microbial pathogens pressure). Using a simulation model, Mouritsen et al. (2005) demonstrated that a 3.8°C increase in ambient temperature would probably result in a parasite‐induced collapse of the amphipod population in the Wadden Sea.

Sensitivity assessment. Sea surface temperatures around the UK are currently between 6-19°C (Huthnance, 2010). Under the three scenarios (middle and high emission and extreme), summer sea temperatures in the south of the UK may rise to temperatures of 22, 23, and 24°C respectively. In the south of the UK, summer air temperatures generally reach a mean maximum of 22°C, and under the three scenarios (middle and high emission and extreme) this temperature is projected to increase to temperatures of 25, 26, and 27°C respectively.

Whilst all three species occur in the intertidal and are able to tolerate a wide range of temperatures, all three of these key characterizing species (Bathyporeia pilosa, Corophium volutator and Corophium arenarium) reach their southern limit at the Bay of Biscay, where summer sea temperatures reach up to 21°C (www.seatemperature.org) and air temperatures reach up to 24°C (www.climate-data.org). It is unknown whether its southern limit is controlled by sea or air temperatures, although as this biotope occurs on the mid and high shore, air temperatures may be more significant, and have been taken into primary consideration for this sensitivity assessment.

Under the middle and high emission scenario, this biotope (LS.LSa.MuSa.BatCare) is expected to experience mean maximum air temperatures of up to 25-26°C, which is greater than what this species experiences at its southerly limit and, therefore, some mortality and potential loss of these species from the south of the UK is possible. Therefore, under these scenarios, resistance has been assessed as ‘Medium’, and resilience has been assessed as ‘Very low’ due to the long-term nature of ocean warming. Therefore, this biotope is assessed as ‘Medium’ sensitivity to ocean warming under the middle and high emission scenarios. Under the extreme scenario, this biotope is predicted to experience mean maximum air temperatures of up to 27°C, and to experience temperatures exceeding those experienced at its southern limit across most of England. Therefore, under this scenario, resistance has been assessed as ‘Low’, whilst resilience has been assessed as ‘Very low’ due to the long term nature of ocean warming. Therefore, this biotope is assessed as having ‘High’ sensitivity to ocean warming under the extreme scenario.

Global warming (high)

High emission scenario (by the end of this century 2081-2100) benchmark of:

• A 4°C rise in SST, NBT (coastal to the shelf seas) and surface air temperature (in eulittoral and supralittoral habitats).

• A 1°C rise in Deep-sea habitats (>200 m) off the continental shelf, and

• A 3°C rise in surface air temperature in intertidal habitats exclusive to Scotland. Further detail.

Evidence

Intertidal species are exposed to extremes of high and low air temperatures during periods of emersion. They must also be able to cope with sharp temperature fluctuations over a short period during the tidal cycle. In winter air temperatures are colder than the sea, conversely in summer air temperatures are much warmer than the sea. Species that occur in the intertidal are, therefore, generally adapted to tolerate a range of temperatures, with the width of the thermal niche positively correlated with the height of the shore that the animal usually occurs (Davenport & Davenport, 2005). The species present in this biotope are present in the mid and/or upper shore and therefore are likely to have broad temperature tolerances and to be tolerant of temperature fluctuations.

The amphipods that occur within this habitat are mobile and can avoid unfavourable conditions to some extent. The life cycle of Bathyporeia spp. vary between locations, which is related to temperature (Mettam, 1989). Preece (1971) tested the temperature tolerance of Bathyporeia pilosa in the laboratory. Individuals acclimated to 15°C for 24 hours were exposed to temperature increases (water temperature raised by 0.2°C/minute). As test temperatures were reached individuals were removed, placed in seawater at 4°C and allowed to recover for 24 hours at which point mortalities were tested. Amphipods were also allowed to bury into sediments and held at test temperatures for 24 hours of 32.5°C, 31.8°C and 29.5°C before being allowed to recover in fresh seawater at 15°C for a further 24 hours, before mortalities were assessed. The upper lethal temperature (the temperature at which 50% of individuals died) for adult males and gravid females of Bathyporeia pilosa was 39.4°C. These tests measures short-term exposure only and species had a lower tolerance for longer-term (24-hour exposure). No mortality occurred for Bathyporeia pilosa individuals held at 29.5°C and 30.8°C. However, 15% of individuals exposed to water temperatures of 31.8°C and 96% at 32.5°C died.

The tolerance of Bathyporeia pilosa to high temperatures may be because it is found in the intertidal, although its ability to withstand long-term changes in temperature is more difficult to discern. Whilst Bathyporeia pilosa can tolerate extremely high temperatures for short periods, this species has a limited distribution from the coast of Norway down to the French coast of the Bay of Biscay (Speybroeck et al., 2008), which suggests that it may be susceptible to ocean warming. As temperatures increase, non-lethal effects of heat stress may lead to changes in population dynamics and its biogeographic range moving northwards to compensate.

Corophium volutator occurs in the North-East Atlantic (NEA) from northern Norway to the Bay of Biscay, and in the North-West Atlantic in the Bay of Fundy and along the coast of Maine (www.obis.org). Whilst it is also thought to be present in the Mediterranean Sea, the Black Sea and the Azov Sea (Neal & Avant, 2006), it is expected that these records refer to Corophium orientale, which is a closely related species originally regarded as a subspecies of Corophium volutator (Crawford, 1937). Corophium arenarium has a distribution from Scotland to the French Coast of the Bay of Biscay. When Corophium volutator was exposed to choice experiments to assess temperature preferences between 0°C and 30°C, it was shown that this species preferred temperatures in the range of 15 - 20°C, although activity peaked at 25°C and it was able to withstand temperatures of 30°C (Meadows & Ruagh, 1981).

Temperature increases may lead to indirect effects on populations of the characterizing species, through changes in the distribution and prevalence of parasites and pathogens (see microbial pathogens pressure). Using a simulation model, Mouritsen et al. (2005) demonstrated that a 3.8°C increase in ambient temperature would probably result in a parasite‐induced collapse of the amphipod population in the Wadden Sea.

Sensitivity assessment. Sea surface temperatures around the UK are currently between 6-19°C (Huthnance, 2010). Under the three scenarios (middle and high emission and extreme), summer sea temperatures in the south of the UK may rise to temperatures of 22, 23, and 24°C respectively. In the south of the UK, summer air temperatures generally reach a mean maximum of 22°C, and under the three scenarios (middle and high emission and extreme) this temperature is projected to increase to temperatures of 25, 26, and 27°C respectively.

Whilst all three species occur in the intertidal and are able to tolerate a wide range of temperatures, all three of these key characterizing species (Bathyporeia pilosa, Corophium volutator and Corophium arenarium) reach their southern limit at the Bay of Biscay, where summer sea temperatures reach up to 21°C (www.seatemperature.org) and air temperatures reach up to 24°C (www.climate-data.org). It is unknown whether its southern limit is controlled by sea or air temperatures, although as this biotope occurs on the mid and high shore, air temperatures may be more significant, and have been taken into primary consideration for this sensitivity assessment.

Under the middle and high emission scenario, this biotope (LS.LSa.MuSa.BatCare) is expected to experience mean maximum air temperatures of up to 25-26°C, which is greater than what this species experiences at its southerly limit and, therefore, some mortality and potential loss of these species from the south of the UK is possible. Therefore, under these scenarios, resistance has been assessed as ‘Medium’, and resilience has been assessed as ‘Very low’ due to the long-term nature of ocean warming. Therefore, this biotope is assessed as ‘Medium’ sensitivity to ocean warming under the middle and high emission scenarios. Under the extreme scenario, this biotope is predicted to experience mean maximum air temperatures of up to 27°C, and to experience temperatures exceeding those experienced at its southern limit across most of England. Therefore, under this scenario, resistance has been assessed as ‘Low’, whilst resilience has been assessed as ‘Very low’ due to the long term nature of ocean warming. Therefore, this biotope is assessed as having ‘High’ sensitivity to ocean warming under the extreme scenario.

Global warming (middle)

Middle emission scenario (by the end of this century 2081-2100) benchmark of:

• A 3°C rise in SST, NBT (coastal to the shelf seas) and surface air temperature (in eulittoral and supralittoral habitats).

• A 1°C rise in Deep-sea habitats (>200 m) off the continental shelf.

• A 2°C rise in surface air temperature in intertidal habitats exclusive to Scotland. Further detail.

Evidence

Intertidal species are exposed to extremes of high and low air temperatures during periods of emersion. They must also be able to cope with sharp temperature fluctuations over a short period during the tidal cycle. In winter air temperatures are colder than the sea, conversely in summer air temperatures are much warmer than the sea. Species that occur in the intertidal are, therefore, generally adapted to tolerate a range of temperatures, with the width of the thermal niche positively correlated with the height of the shore that the animal usually occurs (Davenport & Davenport, 2005). The species present in this biotope are present in the mid and/or upper shore and therefore are likely to have broad temperature tolerances and to be tolerant of temperature fluctuations.

The amphipods that occur within this habitat are mobile and can avoid unfavourable conditions to some extent. The life cycle of Bathyporeia spp. vary between locations, which is related to temperature (Mettam, 1989). Preece (1971) tested the temperature tolerance of Bathyporeia pilosa in the laboratory. Individuals acclimated to 15°C for 24 hours were exposed to temperature increases (water temperature raised by 0.2°C/minute). As test temperatures were reached individuals were removed, placed in seawater at 4°C and allowed to recover for 24 hours at which point mortalities were tested. Amphipods were also allowed to bury into sediments and held at test temperatures for 24 hours of 32.5°C, 31.8°C and 29.5°C before being allowed to recover in fresh seawater at 15°C for a further 24 hours, before mortalities were assessed. The upper lethal temperature (the temperature at which 50% of individuals died) for adult males and gravid females of Bathyporeia pilosa was 39.4°C. These tests measures short-term exposure only and species had a lower tolerance for longer-term (24-hour exposure). No mortality occurred for Bathyporeia pilosa individuals held at 29.5°C and 30.8°C. However, 15% of individuals exposed to water temperatures of 31.8°C and 96% at 32.5°C died.

The tolerance of Bathyporeia pilosa to high temperatures may be because it is found in the intertidal, although its ability to withstand long-term changes in temperature is more difficult to discern. Whilst Bathyporeia pilosa can tolerate extremely high temperatures for short periods, this species has a limited distribution from the coast of Norway down to the French coast of the Bay of Biscay (Speybroeck et al., 2008), which suggests that it may be susceptible to ocean warming. As temperatures increase, non-lethal effects of heat stress may lead to changes in population dynamics and its biogeographic range moving northwards to compensate.

Corophium volutator occurs in the North-East Atlantic (NEA) from northern Norway to the Bay of Biscay, and in the North-West Atlantic in the Bay of Fundy and along the coast of Maine (www.obis.org). Whilst it is also thought to be present in the Mediterranean Sea, the Black Sea and the Azov Sea (Neal & Avant, 2006), it is expected that these records refer to Corophium orientale, which is a closely related species originally regarded as a subspecies of Corophium volutator (Crawford, 1937). Corophium arenarium has a distribution from Scotland to the French Coast of the Bay of Biscay. When Corophium volutator was exposed to choice experiments to assess temperature preferences between 0°C and 30°C, it was shown that this species preferred temperatures in the range of 15 - 20°C, although activity peaked at 25°C and it was able to withstand temperatures of 30°C (Meadows & Ruagh, 1981).

Temperature increases may lead to indirect effects on populations of the characterizing species, through changes in the distribution and prevalence of parasites and pathogens (see microbial pathogens pressure). Using a simulation model, Mouritsen et al. (2005) demonstrated that a 3.8°C increase in ambient temperature would probably result in a parasite‐induced collapse of the amphipod population in the Wadden Sea.

Sensitivity assessment. Sea surface temperatures around the UK are currently between 6-19°C (Huthnance, 2010). Under the three scenarios (middle and high emission and extreme), summer sea temperatures in the south of the UK may rise to temperatures of 22, 23, and 24°C respectively. In the south of the UK, summer air temperatures generally reach a mean maximum of 22°C, and under the three scenarios (middle and high emission and extreme) this temperature is projected to increase to temperatures of 25, 26, and 27°C respectively.

Whilst all three species occur in the intertidal and are able to tolerate a wide range of temperatures, all three of these key characterizing species (Bathyporeia pilosa, Corophium volutator and Corophium arenarium) reach their southern limit at the Bay of Biscay, where summer sea temperatures reach up to 21°C (www.seatemperature.org) and air temperatures reach up to 24°C (www.climate-data.org). It is unknown whether its southern limit is controlled by sea or air temperatures, although as this biotope occurs on the mid and high shore, air temperatures may be more significant, and have been taken into primary consideration for this sensitivity assessment.

Under the middle and high emission scenario, this biotope (LS.LSa.MuSa.BatCare) is expected to experience mean maximum air temperatures of up to 25-26°C, which is greater than what this species experiences at its southerly limit and, therefore, some mortality and potential loss of these species from the south of the UK is possible. Therefore, under these scenarios, resistance has been assessed as ‘Medium’, and resilience has been assessed as ‘Very low’ due to the long-term nature of ocean warming. Therefore, this biotope is assessed as ‘Medium’ sensitivity to ocean warming under the middle and high emission scenarios. Under the extreme scenario, this biotope is predicted to experience mean maximum air temperatures of up to 27°C, and to experience temperatures exceeding those experienced at its southern limit across most of England. Therefore, under this scenario, resistance has been assessed as ‘Low’, whilst resilience has been assessed as ‘Very low’ due to the long term nature of ocean warming. Therefore, this biotope is assessed as having ‘High’ sensitivity to ocean warming under the extreme scenario.

Marine heatwaves (high)

High emission scenario benchmark: A marine heatwave occurring every two years, with a mean duration of 120 days, and a maximum intensity of 3.5°C. Further detail.

Evidence

Marine heatwaves due to increased air-sea heat flux are predicted to occur more frequently, last for longer and at increased intensity by the end of this century under both middle and high emission scenarios (Frölicher et al., 2018). Both Bathyporeia pilosa and Corophium volutator are known to be able to withstand temperatures of 30°C (see Global Warming). Upper lethal temperatures (the temperature at which 50 % of individuals died) for adult males and gravid females of Bathyporeia pilosa were 39.4°C.

Many intertidal species can alter metabolic activity, burrow deeper in sediment or move further down the shore to tolerate or escape unfavourable conditions. Bathyporeia pilosa burrows to a depth of 10 cm (Nicolaisen & Kanneworff, 1969), whilst Corophium volutator and Corophium arenarium have burrows with mean depths of 4.35 cm and 5.27 cm respectively (Jensen & Kristensen, 1990), although sediment temperatures in the first few centimetres of sediment can exceed air temperatures (Harrison & Phizacklea, 1987). At low tide, air temperature becomes critically important to intertidal animals, and on sandy beaches, the substratum, from the surface to a depth of several centimetres, can experience large variations in temperature during a single cycle and throughout the year (Harrison & Phizacklea, 1987; Hayward, 1994). For instance, Khayrallah & Jones (1980b) reported the temperature range of sand at a depth of 1 cm during neap tides to be from -2°C in February 1973, to a maximum of 25°C in July 1977. Although adapted to temperature change, extremely warm temperatures that occur during a heatwave may result in a seasonal reduction in species richness and abundance. Negative impacts of heatwaves may not only be related to temperature stress but may be related to the resultant changes in other factors, especially reductions in oxygen resulting from enhanced microbial activity (Hayward, 1994; Eltringham, 1971).

Under the middle emission scenario, if heatwaves occurred every three years, with a maximum intensity of 2°C for 80 days by the end of this century, this could lead to summer sea temperatures reaching up to 24°C in southern England, whilst mean maximum air temperatures could reach 27°C. These temperatures are higher than the characterizing species of this biotope experience at their southern limit in the Bay of Biscay (see Global Warming), although both these species have been shown to be able to tolerate short term temperatures of ≥ 30°C. Therefore, some mortality cannot be ruled out, although recovery is likely to be rapid. Resistance has been assessed as ‘Medium’ and recovery has been assessed as ‘High’, leading to an assessment of ‘Low’ for this biotope under the middle emission scenario.

Under the high emission scenario, if heatwaves occur every two years by the end of this century, reaching a maximum intensity of 3.5°C for 120 days, this could lead to the heatwave lasting the entire summer with sea temperatures reaching up to 26.5°C, and mean maximum air temperatures exceeding 30°C across the south of the UK. Under this scenario, the characterizing species of this biotope may suffer significant mortality through acute physiological stress and, therefore, resistance has been assessed as ‘Low’. These species are known to have long reproductive seasons during their short lifespans, and as some individuals remain, recovery is likely to be rapid. Resilience has been assessed as ‘High’, leading to a sensitivity assessment of ‘Low’ under the high emission scenario.

Marine heatwaves (middle)

Middle emission scenario benchmark:  A marine heatwave occurring every three years, with a mean duration of 80 days, with a maximum intensity of 2°C. Further detail.

Evidence

Marine heatwaves due to increased air-sea heat flux are predicted to occur more frequently, last for longer and at increased intensity by the end of this century under both middle and high emission scenarios (Frölicher et al., 2018). Both Bathyporeia pilosa and Corophium volutator are known to be able to withstand temperatures of 30°C (see Global Warming). Upper lethal temperatures (the temperature at which 50 % of individuals died) for adult males and gravid females of Bathyporeia pilosa were 39.4°C.

Many intertidal species can alter metabolic activity, burrow deeper in sediment or move further down the shore to tolerate or escape unfavourable conditions. Bathyporeia pilosa burrows to a depth of 10 cm (Nicolaisen & Kanneworff, 1969), whilst Corophium volutator and Corophium arenarium have burrows with mean depths of 4.35 cm and 5.27 cm respectively (Jensen & Kristensen, 1990), although sediment temperatures in the first few centimetres of sediment can exceed air temperatures (Harrison & Phizacklea, 1987). At low tide, air temperature becomes critically important to intertidal animals, and on sandy beaches, the substratum, from the surface to a depth of several centimetres, can experience large variations in temperature during a single cycle and throughout the year (Harrison & Phizacklea, 1987; Hayward, 1994). For instance, Khayrallah & Jones (1980b) reported the temperature range of sand at a depth of 1 cm during neap tides to be from -2°C in February 1973, to a maximum of 25°C in July 1977. Although adapted to temperature change, extremely warm temperatures that occur during a heatwave may result in a seasonal reduction in species richness and abundance. Negative impacts of heatwaves may not only be related to temperature stress but may be related to the resultant changes in other factors, especially reductions in oxygen resulting from enhanced microbial activity (Hayward, 1994; Eltringham, 1971).

Under the middle emission scenario, if heatwaves occurred every three years, with a maximum intensity of 2°C for 80 days by the end of this century, this could lead to summer sea temperatures reaching up to 24°C in southern England, whilst mean maximum air temperatures could reach 27°C. These temperatures are higher than the characterizing species of this biotope experience at their southern limit in the Bay of Biscay (see Global Warming), although both these species have been shown to be able to tolerate short term temperatures of ≥ 30°C. Therefore, some mortality cannot be ruled out, although recovery is likely to be rapid. Resistance has been assessed as ‘Medium’ and recovery has been assessed as ‘High’, leading to an assessment of ‘Low’ for this biotope under the middle emission scenario.

Under the high emission scenario, if heatwaves occur every two years by the end of this century, reaching a maximum intensity of 3.5°C for 120 days, this could lead to the heatwave lasting the entire summer with sea temperatures reaching up to 26.5°C, and mean maximum air temperatures exceeding 30°C across the south of the UK. Under this scenario, the characterizing species of this biotope may suffer significant mortality through acute physiological stress and, therefore, resistance has been assessed as ‘Low’. These species are known to have long reproductive seasons during their short lifespans, and as some individuals remain, recovery is likely to be rapid. Resilience has been assessed as ‘High’, leading to a sensitivity assessment of ‘Low’ under the high emission scenario.

Ocean acidification (high)

High emission scenario benchmark: a further decrease in pH of 0.35 (annual mean) and corresponding 120% increase in H+ ions , seasonal aragonite saturation of 20% of UK coastal waters and North Sea bottom waters, and the aragonite saturation horizon in the NE Atlantic, off the continental shelf, occurring at a depth of 400 m by the end of this century 2081-2100. Further detail 

Evidence

Amphipods are generally thought to be less sensitive to ocean acidification than some other taxa and are found in greater numbers at naturally CO₂ enriched vents (Kroeker et al., 2011, Garrard et al., 2014, Vizzini et al., 2017). This increase in abundance is not directly related to CO₂ enrichment, but rather due to indirect effects such as reduced predation or increased food supply.  A laboratory study found that under CO₂ enrichment, the population size of the amphipod Gammarus locusta increased 20 fold and the proportion of gravid females doubled, suggesting that ocean acidification may confer an advantage to amphipods by relaxing environmental constraints on reproduction (Heldt et al., 2016). Further laboratory experiments show little effect of ocean acidification at levels expected for the high emission scenario at the end of this century (pH 7.8) (Hauton et al., 2009, Hale et al., 2011, Lim & Harley, 2018).

Increasing levels of CO₂ in the atmosphere have led to the average pH of sea surface waters dropping from 8.25 in the 1700s to 8.14 in the 1990s (Jacobson, 2005). When exposed experimentally to CO₂ enrichment that led to a decrease in pH of 0.2 units and 0.34 units, Corophium volutator showed no mortality, although at the higher pH decrease (0.34 units) there was a significant increase in DNA damage from 10 - 17 % (Roberts et al., 2013). There is no direct evidence of the impact of ocean acidification on amphipods from the genus Bathyporeia.

Sensitivity Assessment. Corophium volutator appears to be generally robust to levels of ocean acidification projected for the end of this century. Although increased DNA damage may lead to some negative effects, this is a short-lived, highly fecund species, and it is unlikely to lead to population-level effects. Direct evidence of the impact of ocean acidification on Bathyporeia pilosa is lacking. However, in general, amphipods appear to be tolerant. Therefore, it is likely that the characterizing species of this biotope will show a ‘High’ resistance to a decrease in pH. Hence, the biotope is assessed as ‘High’ resistance to ocean acidification, based on the evidence available under both the middle and high emission scenarios. Resilience is assessed as ‘High’ leading to an assessment of ‘Not sensitive’ at the benchmark level.

Ocean acidification (middle)

Middle emission scenario benchmark: a further decrease in pH of 0.15 (annual mean) and corresponding 35% increase in H+ ions with no coastal aragonite undersaturation and the aragonite saturation horizon in the NE Atlantic, off the continental shelf, at a depth of 800 m by the end of this century 2081-2100. Further detail.

Evidence

Amphipods are generally thought to be less sensitive to ocean acidification than some other taxa and are found in greater numbers at naturally CO₂ enriched vents (Kroeker et al., 2011, Garrard et al., 2014, Vizzini et al., 2017). This increase in abundance is not directly related to CO₂ enrichment, but rather due to indirect effects such as reduced predation or increased food supply.  A laboratory study found that under CO₂ enrichment, the population size of the amphipod Gammarus locusta increased 20 fold and the proportion of gravid females doubled, suggesting that ocean acidification may confer an advantage to amphipods by relaxing environmental constraints on reproduction (Heldt et al., 2016). Further laboratory experiments show little effect of ocean acidification at levels expected for the high emission scenario at the end of this century (pH 7.8) (Hauton et al., 2009, Hale et al., 2011, Lim & Harley, 2018).

Increasing levels of CO₂ in the atmosphere have led to the average pH of sea surface waters dropping from 8.25 in the 1700s to 8.14 in the 1990s (Jacobson, 2005). When exposed experimentally to CO₂ enrichment that led to a decrease in pH of 0.2 units and 0.34 units, Corophium volutator showed no mortality, although at the higher pH decrease (0.34 units) there was a significant increase in DNA damage from 10 - 17 % (Roberts et al., 2013). There is no direct evidence of the impact of ocean acidification on amphipods from the genus Bathyporeia.

Sensitivity Assessment. Corophium volutator appears to be generally robust to levels of ocean acidification projected for the end of this century. Although increased DNA damage may lead to some negative effects, this is a short-lived, highly fecund species, and it is unlikely to lead to population-level effects. Direct evidence of the impact of ocean acidification on Bathyporeia pilosa is lacking. However, in general, amphipods appear to be tolerant. Therefore, it is likely that the characterizing species of this biotope will show a ‘High’ resistance to a decrease in pH. Hence, the biotope is assessed as ‘High’ resistance to ocean acidification, based on the evidence available under both the middle and high emission scenarios. Resilience is assessed as ‘High’ leading to an assessment of ‘Not sensitive’ at the benchmark level.

Sea level rise (extreme)

Extreme scenario benchmark: a 107 cm rise in average UK by the end of this century (2018-2100). Further detail.

Evidence

A rise in sea level increases the water depth at the shore and results in increased wave and tidal energy along the shore, due to the increase in fetch and reduction in wave attenuation (Pethick, 2001; Crooks, 2004; Fujii, 2012).  As a result, coast landforms (e.g. subtidal bedforms, intertidal flats, saltmarshes, shingle banks, sand dunes, cliffs and coastal lowlands) migrate along and parallel to the shore to maintain their position within the coastal energy gradient (Cooks, 2004; Fujii, 2012).  For example, mudflats migrate landwards to a lower energy position and may be replaced by sand flats that have themselves migrated landwards from exposed conditions (Crooks, 2004).  In effect, ‘coastal roll-over’ results as the shore moves landwards by the erosion of the landward, upper limit, of the shore and deposition at its lower limit (Crooks, 2004).  Pethick (2001) suggested that a sea-level rise rate of 6 mm/yr.could result in landward movement of estuaries by 10 m/yr., long-shore migration of open coast landforms of 50 m/yr. and ebb-tidal deltas to extend laterally by 300 m/yr.  Of course, there is no guarantee that landward migration to compensate for sea-level rise will be possible.

The effects of sea-level rise and increased wave action increase further due to storms and storm surges. IPCC (2019) note that the frequency of extreme sea-level events (e.g. due to storms) are predicted to increase as sea-level rises, however, there is no consensus on the future storm and, hence, wave climate around UK coasts (Mossman et al., 2015; Lowe et al., 2018; Palmer et al., 2018).

The LS.LSa.MuSa.BatCare biotope occurs most commonly on medium to muddy sand on the upper and mid-shore of broad estuaries (JNCC, 2015). Therefore, an increase in sea level height of 50, 70 and 107 cm could have severe repercussions for the extent of this biotope. Beds may be able to expand their range and migrate landwards to compensate for sea-level rise, if not constrained by lack of suitable habitat or human modification of the environment (IPCC, 2019). If landward migration is not possible, it is expected that depth distribution of Bathyporeia pilosa and Corophium arenarium in littoral muddy sand will shrink in response to a 50, 70 or 107 cm sea-level rise, without the possibility of recovery. The possibility to migrate inshore will be site-specific, hence, the assessment is based on a worst-case-scenario, i.e. that landward migration is not possible.

Sensitivity assessment. The mean tidal range in the UK varies from 127 cm in the Shetland Islands to 972 cm at Avonmouth, in the Bristol Channel (Woodworth et al., 1991). This large difference in tidal amplitudes suggests that this biotope will be more affected in some parts of the UK than others. In Scotland and Ireland, where mean tidal range is generally less than 3 m (Woodworth et al., 1991), a third of this biotope may be lost under the extreme scenario, whereas in the Bristol Channel, where mean tidal range exceeds 9 m (Woodworth et al., 1991), only a small portion of this biotope may be lost. Under the medium and high emission scenarios, resistance has been assessed as ‘Medium’, as less than 25% of this biotope could be lost. Resilience has been assessed as ‘Very low’, due to the long-term nature of sea-level rise.  Therefore, sensitivity is assessed as ‘Medium’.  Under the high emission and extreme scenarios, resistance has been assessed as ‘Low’, as it is possible that more than 25% of this biotope could be lost. Resilience has been assessed as ‘Very low’, due to the long-term nature of sea-level rise.  Therefore, sensitivity is assessed as ‘High’, albeit with ‘Low’ confidence.

Sea level rise (high)

High emission scenario benchmark: a 70 cm rise in average UK by the end of this century (2018-2100). Further detail.

Evidence

A rise in sea level increases the water depth at the shore and results in increased wave and tidal energy along the shore, due to the increase in fetch and reduction in wave attenuation (Pethick, 2001; Crooks, 2004; Fujii, 2012).  As a result, coast landforms (e.g. subtidal bedforms, intertidal flats, saltmarshes, shingle banks, sand dunes, cliffs and coastal lowlands) migrate along and parallel to the shore to maintain their position within the coastal energy gradient (Cooks, 2004; Fujii, 2012).  For example, mudflats migrate landwards to a lower energy position and may be replaced by sand flats that have themselves migrated landwards from exposed conditions (Crooks, 2004).  In effect, ‘coastal roll-over’ results as the shore moves landwards by the erosion of the landward, upper limit, of the shore and deposition at its lower limit (Crooks, 2004).  Pethick (2001) suggested that a sea-level rise rate of 6 mm/yr.could result in landward movement of estuaries by 10 m/yr., long-shore migration of open coast landforms of 50 m/yr. and ebb-tidal deltas to extend laterally by 300 m/yr.  Of course, there is no guarantee that landward migration to compensate for sea-level rise will be possible.

The effects of sea-level rise and increased wave action increase further due to storms and storm surges. IPCC (2019) note that the frequency of extreme sea-level events (e.g. due to storms) are predicted to increase as sea-level rises, however, there is no consensus on the future storm and, hence, wave climate around UK coasts (Mossman et al., 2015; Lowe et al., 2018; Palmer et al., 2018).

The LS.LSa.MuSa.BatCare biotope occurs most commonly on medium to muddy sand on the upper and mid-shore of broad estuaries (JNCC, 2015). Therefore, an increase in sea level height of 50, 70 and 107 cm could have severe repercussions for the extent of this biotope. Beds may be able to expand their range and migrate landwards to compensate for sea-level rise, if not constrained by lack of suitable habitat or human modification of the environment (IPCC, 2019). If landward migration is not possible, it is expected that depth distribution of Bathyporeia pilosa and Corophium arenarium in littoral muddy sand will shrink in response to a 50, 70 or 107 cm sea-level rise, without the possibility of recovery. The possibility to migrate inshore will be site-specific, hence, the assessment is based on a worst-case-scenario, i.e. that landward migration is not possible.

Sensitivity assessment. The mean tidal range in the UK varies from 127 cm in the Shetland Islands to 972 cm at Avonmouth, in the Bristol Channel (Woodworth et al., 1991). This large difference in tidal amplitudes suggests that this biotope will be more affected in some parts of the UK than others. In Scotland and Ireland, where mean tidal range is generally less than 3 m (Woodworth et al., 1991), a third of this biotope may be lost under the extreme scenario, whereas in the Bristol Channel, where mean tidal range exceeds 9 m (Woodworth et al., 1991), only a small portion of this biotope may be lost. Under the medium and high emission scenarios, resistance has been assessed as ‘Medium’, as less than 25% of this biotope could be lost. Resilience has been assessed as ‘Very low’, due to the long-term nature of sea-level rise.  Therefore, sensitivity is assessed as ‘Medium’.  Under the high emission and extreme scenarios, resistance has been assessed as ‘Low’, as it is possible that more than 25% of this biotope could be lost. Resilience has been assessed as ‘Very low’, due to the long-term nature of sea-level rise.  Therefore, sensitivity is assessed as ‘High’, albeit with ‘Low’ confidence.

Sea level rise (middle)

Middle emission scenario benchmark: a 50 cm rise in average UK sea-level rise by the end of this century (2081-2100). Further detail.

Evidence

A rise in sea level increases the water depth at the shore and results in increased wave and tidal energy along the shore, due to the increase in fetch and reduction in wave attenuation (Pethick, 2001; Crooks, 2004; Fujii, 2012).  As a result, coast landforms (e.g. subtidal bedforms, intertidal flats, saltmarshes, shingle banks, sand dunes, cliffs and coastal lowlands) migrate along and parallel to the shore to maintain their position within the coastal energy gradient (Cooks, 2004; Fujii, 2012).  For example, mudflats migrate landwards to a lower energy position and may be replaced by sand flats that have themselves migrated landwards from exposed conditions (Crooks, 2004).  In effect, ‘coastal roll-over’ results as the shore moves landwards by the erosion of the landward, upper limit, of the shore and deposition at its lower limit (Crooks, 2004).  Pethick (2001) suggested that a sea-level rise rate of 6 mm/yr.could result in landward movement of estuaries by 10 m/yr., long-shore migration of open coast landforms of 50 m/yr. and ebb-tidal deltas to extend laterally by 300 m/yr.  Of course, there is no guarantee that landward migration to compensate for sea-level rise will be possible.

The effects of sea-level rise and increased wave action increase further due to storms and storm surges. IPCC (2019) note that the frequency of extreme sea-level events (e.g. due to storms) are predicted to increase as sea-level rises, however, there is no consensus on the future storm and, hence, wave climate around UK coasts (Mossman et al., 2015; Lowe et al., 2018; Palmer et al., 2018).

The LS.LSa.MuSa.BatCare biotope occurs most commonly on medium to muddy sand on the upper and mid-shore of broad estuaries (JNCC, 2015). Therefore, an increase in sea level height of 50, 70 and 107 cm could have severe repercussions for the extent of this biotope. Beds may be able to expand their range and migrate landwards to compensate for sea-level rise, if not constrained by lack of suitable habitat or human modification of the environment (IPCC, 2019). If landward migration is not possible, it is expected that depth distribution of Bathyporeia pilosa and Corophium arenarium in littoral muddy sand will shrink in response to a 50, 70 or 107 cm sea-level rise, without the possibility of recovery. The possibility to migrate inshore will be site-specific, hence, the assessment is based on a worst-case-scenario, i.e. that landward migration is not possible.

Sensitivity assessment. The mean tidal range in the UK varies from 127 cm in the Shetland Islands to 972 cm at Avonmouth, in the Bristol Channel (Woodworth et al., 1991). This large difference in tidal amplitudes suggests that this biotope will be more affected in some parts of the UK than others. In Scotland and Ireland, where mean tidal range is generally less than 3 m (Woodworth et al., 1991), a third of this biotope may be lost under the extreme scenario, whereas in the Bristol Channel, where mean tidal range exceeds 9 m (Woodworth et al., 1991), only a small portion of this biotope may be lost. Under the medium and high emission scenarios, resistance has been assessed as ‘Medium’, as less than 25% of this biotope could be lost. Resilience has been assessed as ‘Very low’, due to the long-term nature of sea-level rise.  Therefore, sensitivity is assessed as ‘Medium’.  Under the high emission and extreme scenarios, resistance has been assessed as ‘Low’, as it is possible that more than 25% of this biotope could be lost. Resilience has been assessed as ‘Very low’, due to the long-term nature of sea-level rise.  Therefore, sensitivity is assessed as ‘High’, albeit with ‘Low’ confidence.

Temperature increase (local)

Benchmark. A 5°C increase in temperature for one month, or 2°C for one year. Further detail

Evidence

Intertidal species are exposed to extremes of high and low air temperatures during periods of emersion. They must also be able to cope with sharp temperature fluctuations over a short period of time during the tidal cycle. In winter air temperatures are colder than the sea, conversely in summer air temperatures are much warmer than the sea. Species that occur in the intertidal are therefore generally adapted to tolerate a range of temperatures, with the width of the thermal niche positively correlated with the height of the shore that the animal usually occurs at (Davenport & Davenport, 2005). The species present in this biotope are present in the mid and/or upper shore and therefore are likely to have broad temperature tolerances and to be tolerant of temperature fluctuations.

Many intertidal species can alter metabolic activity, burrow deeper in sediment or move to deeper water to tolerate or escape unfavourable conditions. At low tide, air temperature becomes critically important to intertidal animals, and on sandy beaches the substratum, from the surface to a depth of several centimetres, can experience large variations in temperature during a single cycle and throughout the year (Hayward, 1994). For instance, Khayrallah & Jones (1980b) reported the temperature range of sand at a depth of 1 cm during neap tides to be from -2°C in February 1973, to a maximum of 25°C in July 1977. Although adapted to temperature change, severe acute change may result in seasonal reduction in species richness and abundance, but the effects of an acute temperature increase are not necessarily direct and may be more related to the resultant changes in other factors, especially reductions in oxygen resulting from enhanced microbial activity (Hayward, 1994; Eltringham, 1971).

The amphipods that occur within this habitat are mobile and can avoid unfavourable conditions to some extent. Bathyporeia life cycles vary between locations and this is related to temperature (Mettam, 1989). Preece (1971) tested temperature tolerances of Bathyporeia pilosa in the laboratory. Individuals acclimated to 15°C for 24 hours were exposed to temperature increases (water temperature raised by 0.2°C/minute). As test temperature were reached individuals were removed, placed in seawater at 4°C and allowed to recover for 24 hours at which point mortalities were tested. Amphipods were also allowed to bury into sediments and held at test temperatures for 24 hours of 32.5°C, 31.8°C and 29.5°C before being allowed to recover in fresh seawater at 15°C for a further 24 hours, before mortalities were assessed. Upper lethal temperatures (the temperature at which 50% of individuals died for adult males and gravid females of Bathyporeia pilosa were 39.4°C. These tests measures short-term exposure only and species had lower tolerance for longer-term (24 hour exposure). No mortality occurred for Bathyporeia pilosa individuals held at 29.5°C and 30.8°C; however 15% of individuals exposed to water temperatures of 31.8°C and 96% at 32.5°C died. 

Corophium volutator is widely distributed in the north Atlantic, American and European coasts, from western Norway to the Mediterranean and the Black Sea and Azov Sea (Neal & Avant, 2006). The amphipod is subject to temperatures of 1°C in the winter to 17°C in the summer (Wilson & Parker, 1996) but can resist much higher temperatures (Meadows & Ruagh, 1981).

Temperature increases may lead to indirect effects on populations of the characterizing species, through changes in the distribution and prevalence of parasites and pathogens (see microbial pathogens pressure). Using a simulation model, Mouritsen et al. (2005) demonstrated that a 3.8 °C increase in ambient temperature would probably result in a parasite‐induced collapse of the amphipod population in the Wadden Sea. This indirect effect is assessed in the introduction of microbial pathogens pressure.

Sensitivity assessment. Typical surface water temperatures around the UK coast vary seasonally from 4-19°C (Huthnance, 2010). A chronic increase in temperature throughout the year of 2°C may fall within the normal temperature variation and an acute increase in water temperatures from 19 to 24°C for a month may be tolerated by the characterizing species supported by deeper burrowing and/or migration. For Bathyporeia spp. temperature increases above 30^oC appear to be critical based on Preece (1971). For intertidal biotopes air temperature may be more critical in the hottest parts of the year although nocturnal emergence of Bathyporeia spp. and isopods or burrowing life habit may also enhance survival. Biotope resistance is therefore assessed as ‘High’ and resilience as ‘High’ so that the biotope is assessed as ‘Not sensitive’. 

Temperature decrease (local)

Benchmark. A 5°C decrease in temperature for one month, or 2°C for one year. Further detail

Evidence

Intertidal species are exposed to extremes of high and low air temperatures during periods of emersion. They must also be able to cope with sharp temperature fluctuations over a short period of time during the tidal cycle. In winter air temperatures are colder than the sea, conversely in summer air temperatures are much warmer than the sea. Species that occur in the intertidal are therefore generally adapted to tolerate a range of temperatures, with the width of the thermal niche positively correlated with the height of the shore that the animal usually occurs at (Davenport & Davenport, 2005).

Crisp (1964) reported that species of amphipod and isopods seemed to be unharmed by the severe winter of 1962-1963. This may be due to burial in sediments buffering temperature or seasonal migration to deeper waters to avoid freezing. In the winter migrations have also been observed for Bathyporeia spp. (Fish & Fish, 1978; Fish & Preece, 1970).

Preece (1971) tested temperature tolerances of Bathyporeia pilosa in the laboratory. Individuals acclimated to 15°C for 24 hours were placed in a freezer in wet sediment. As test temperatures were reached individuals were removed and allowed to recover for 24 hours at which point mortalities were tested. Amphipods were also allowed to bury into sediments and held at test temperatures of -1°C, -3°C and -5°C for 24 hours before being allowed to recover in fresh seawater at 15°C for a further 24 hours before mortalities were assessed. The lower lethal short-term tolerances of Bathyporeia pilosa were -13.6°C. Bathyporeia pilosa individuals could withstand temperatures as low as -1°C for 24 hours, at -3°C, 5% of Bathyporeia pilosa died but this rose to 82% at -5°C.

Corophium volutator is subject to temperatures of 1°C in the winter to 17°C in the summer (Wilson & Parker, 1996). The population may reduce activity and delay reproduction if the temperature drops below 7°C. Sudden pulses of very cold water can disrupt the circa-tidal rhythms of Corophium volutator by resetting the onset of swimming behaviour. For example, a 6 hour cold spell would lead to the population trying to swim at low tide and leave them vulnerable to increased predation. However, it took temperatures of 15-20°C below ambient temperature to induce this response (Holmström & Morgan, 1983b).

Drolet et al. (2013) sampled two intertidal mudflats in the upper Bay of Fundy, Canada, over two consecutive winters (2009–2011), where sediment temperature, 5 cm deep, reached −2°C. The authors concluded Corophium volutator survived winter through  physiological adaptations.  

Sensitivity assessment. Typical surface water temperatures around the UK coast vary seasonally from 4-19°C (Huthnance, 2010). A chronic decrease in temperature throughout the year of 2°C may fall within the normal temperature variation.  An acute decrease in water temperatures from 4°C to -1°C at the coldest part of the year may lead to freezing and lethal effects on exposed individuals but the characterizing species may survive by burrowing deeper into sediments and/or migration. For Bathyporeia pilosa seawater temperature decreases below -1°C appear to be critical based on Preece (1971). For intertidal biotopes air temperature may be more important than seawater temperatures in the coldest parts of the year and nocturnal emergence of Bathyporeia pilosa may reduce survival although these species are more likely to be quiescent during cold periods or to have migrated to the subtidal. Biotope resistance is therefore assessed as ‘High’ and resilience as ‘High’ so that the biotope is assessed as ‘Not sensitive’. 

Salinity increase (local)

Benchmark. A increase in one MNCR salinity category above the usual range of the biotope or habitat. Further detail

Evidence

This biotope is found in full salinity (30-35 ppt) and variable salinity habitats (18-35 ppt) (JNCC, 2015). Biotope examples found in variable salinity are considered to tolerate a change to full salinity as this falls within the natural range. A change at the pressure benchmark is therefore assessed as a change to hypersaline conditions (>40 ppt) from full salinity. Little evidence was found to assess responses to hypersalinity. However, monitoring at a Spanish desalination facility where discharges close to the outfall reached a salinity of 53, found that amphipods were sensitive to the increased salinity and that species free-living in the sediment were most sensitive. The study area did not host any of the species characterizing this biotope but the results indicate a general sensitivity (De-la-Ossa-Carretero et al., 2016).

Bathyporeia pilosa appears to be confined to intertidal sand sediments. Corophium volutator occupies a greater variety of habitats and is found in fine sediments of mud flats, salt marsh pools and brackish ditches. It is therefore exposed in its natural range to salinity extremes, from freshwater flushing during periods of high rainfall and increases in salinity during periods of high evaporation. Corophium volutator is, therefore, an exceptionally euryhaline species able to tolerate 2-50 psu (McLusky, 1968) but growth is fastest at 15-20 psu (McLusky, 1967; McLusky, 1970 cited in Meadows & Ruagh, 1981). The interstitial salinity is more important for Corophium volutator than that of the overlying water. Sustained periods of increased salinity are required to alter that of the interstitial water and there is a lag between salinity changes and the response of Corophium volutator (McLusky, 1968). Sudden increases in salinity delay swimming activity (Harris & Morgan, 1984a). Corophium volutator will also migrate from areas of unfavourable salinity (McLusky, 1968). Corophium arenarium is more tolerant of Corophium volutator to short-term increases in salinity <45‰ (Mills & Fish, 1980).

Sensitivity assessment: The characterizing species of this biotope are euryhaline and likely to be resistant of an increase in salinity. However, a chronic increase at the pressure benchmark level is likely to result in the death of a portion of the individuals in the population. Resistance is therefore assessed as ‘Low’ and resilience is likely to be ‘High’ so the biotope is considered to have ‘Low’ sensitivity to an increase in salinity at the pressure benchmark level.

Salinity decrease (local)

Benchmark. A decrease in one MNCR salinity category above the usual range of the biotope or habitat. Further detail

Evidence

This biotope is found in full salinity (30-35 ppt) and variable salinity habitats (18-35 ppt) (JNCC, 2015). A change at the pressure benchmark refers to a change to low (<18 ppt salinity).  

Speybroeck et al. (2008) noted that Bathyporeia pilosa tends to occur subtidally in estuarine and brackish conditions. Bathyporeia pilosa is tolerant of low salinities and it is capable of reproducing at salinities as low as 2 (Khayrallah, 1977). Populations of Bathyporeia pilosa within the upper reaches of the Severn Estuary experience wide fluctuations in salinity ranging from 1-22 depending on the season and tidal cycle (Mettam, 1989). The physiological stress resulting from this environment, however, affects size and reproduction (Mettam, 1989).

Corophium volutator is an exceptionally euryhaline species able to tolerate 2-50 psu (McLusky, 1968) but growth is fastest at 15-20 psu (McLusky, 1970 cited in Meadows & Ruagh, 1981). Corophium volutator requires a salinity greater than 5 psu in order to moult, since osmoregulation is lost during moulting (McLusky, 1967). A salinity of at least 7.5 psu is required for reproduction (McLusky, 1968). Mills & Fish (1980), found that Corophium volutator is more tolerant of low salinity (2 to 10‰) than Corophium arenarium. Females undergoing a pre-copulatory moult failed to lay eggs below salinities of 3‰ (Corophium volutator) and 10‰ (Corophium arenarium), but in both species the lowest salinity at which all females moulted and laid eggs was 20‰. Changes in salinity are very unlikely to cause mortality but may alter population distribution and abundances within the biotope as the species is likely to move to more favourable conditions or to suffer some effects on reproduction with resulting decreases in abundance.

Sensitivity assessment: Both the characterizing Bathyporeia pilosa and Corophium spp. are likely to resist a decrease in salinity at the pressure benchmark level, although there may be some decreases in abundance and body size due to physiological stress, depending on the degree of decrease. Resistance is therefore assessed as ‘Medium’ and resilience as ‘High’ (following restoration of the usual salinity regime), and biotope sensitivity is assessed as ‘Low’.

Water flow (tidal current) changes (local)

Benchmark. A change in peak mean spring bed flow velocity of between 0.1 m/s to 0.2 m/s for more than one year. Further detail

Evidence

Water movement from tidal streams is a key factor physically structuring this biotope (JNCC, 2015). Sediment sorting and mobilization by tidal streams modifies the sediments present and creates a suitable habitat for Bathyporeia pilosa that is more typical of wave disturbed habitats. A change in water flow at the pressure benchmark may be significant as sand fractions are not cohesive (particularly where these occur higher on the shore and are coarser and drier) and are eroded at lower current velocities that muds and silts. Sand particles are most likely to be eroded at about 0.20 m/s (based on the Hjulström-Sundborg diagram, Sundborg, 1956).

Bathyporeia spp. are tolerant of disturbed and mobile sandy sediments and can re-position and burrow. They are therefore unlikely to be impacted by an increase at the pressure benchmark even if this results in more sediment disturbance and a change to coarser sands. Changes in sediment from sands to muddy sands following increased deposition or a change from muddy sand to clean sand may alter abundances and distribution of Corophium volutator and Corophium arenarium due to their differing sediment preferences (see physical change pressures). Decreases in flow rate are not considered to negatively impact Corophium volutator which inhabits muddy sediments in low energy environments such as saltmarsh pools.  

Sensitivity assessment. The sediments that characterize this biotope and sub-biotopes are mobile medium to fine sands and sometimes muddy sands. A change at the pressure benchmark (increase or decrease) may lead to some changes in sediment sorting. However, it is unlikely that a decrease at the pressure benchmark would lead to the development of a mud sediment as fine silts require lower velocities to deposit than erode. An increase at the pressure benchmark may lead to increased erosion of fine silts but the biotope is likely to persist and some sediment restoration is likely during the slower neap tides. Biotope resistance is therefore assessed as ‘High’ and resilience as ‘High’ (by default), so that the biotope is assessed as ‘Not sensitive’. 

Emergence regime changes

Benchmark.  1) A change in the time covered or not covered by the sea for a period of ≥1 year or 2) an increase in relative sea level or decrease in high water level for ≥1 year. Further detail

Evidence

This biotope is found on the lower, mid and upper shore (JNCC, 2015). On sandy shores (as in rocky shores) zonation of species occurs in relation to tidal height, in general, talitrid amphipods tend to occur higher on the shore where sediments dry out at low tide and macroalgae is deposited, while lower down the shore more species rich assemblages occur and suspension feeding bivalves are more abundant in the damper sediments (McLachlan et al., 1995). The zonation pattern is mediated by local hydrodynamics and the associated sediment characteristics. This biotope is typically found higher up the shore than sandflats with the cockle Cerastoderma edule in the large sandy estuaries of the west coast of England and Wales. In moderately exposed conditions, this biotope can occur on the mid shore below LS.LSa.St.Tal and/or LS.LSa.MoSa. In more sheltered conditions, this biotope may occur above LS.LMu.MEst.NhomLimStr (JNCC, 2015).

Changes in emergence are likely to result in some shifts in the shoreward or landward extent of the biotope group due to changes in the length of time the biotope is uncovered and covered. Decreased emergence could lead to biotopes occurring on the lower shore becoming sub-littoral or colonized by bivalves and more species rich assemblages. Similarly upper shore biotopes that are at the limit of the ebbing tide could revert to strandline biotopes LS.LSa.St.Tal or revert to the species-poor biotope LS.LSa.MoSa or the Scolelepis squamata dominated variant (LS.LSa.MoSa.AmSco.Sco) due to that species tolerance for well-drained habitats

Sensitivity assessment. Sandy beaches exhibit vertical zonation of biotopes due to species differing tolerances for emergence. Species within the biotope exhibit physiological and behavioural adaptations to changes and the biotope as a group is unlikely to be very sensitive to changes in emergence that do not alter the shore zone (from mid-eulittoral to supralittoral, for example). Changes in emergence may result in some changes in upper and lower extents of the biotope and the sub-biotopes and biotope resistance is therefore assessed as ‘Medium’, resilience is assessed as ‘High’ (following restoration of tidal regime) and biotope sensitivity is therefore ‘Low’. 

Wave exposure changes (local)

Benchmark. A change in near shore significant wave height of >3% but <5% for more than one year. Further detail

Evidence

This biotope occurrs in a range of estimated wave exposures, including; moderately exposed, sheltered, very sheltered and extremely sheltered (JNCC, 2015). Water movement is an important factor that physically structures this biotope through sediment sorting and disturbance, but tidal streams rather than wave action are the key element (JNCC, 2015).

Sensitivity assessment. As the biotope occurs across four wave exposure categories (JNCC, 2015) this is considered to indicate, by proxy, that a change in wave exposure at the pressure benchmark is less than the natural range of wave heights experienced, (particularly as wave height and hence the corresponding change will be small). Biotope resistance to this pressure is therefore assessed as ‘High’ and resilience as ‘High (by default) so that the biotope is considered to be ‘Not sensitive’ at the pressure benchmark.

Transition elements & organo-metal contamination

Benchmark. Exposure of marine species or habitat to one or more relevant contaminants via uncontrolled releases or incidental spills. Further detail

Evidence

This pressure is Not assessed but evidence is presented where available.

Levels of contaminants that exceed the pressure benchmark may cause impacts. For most metals, toxicity to crustaceans increases with decreased salinity and elevated temperature, therefore marine species living within their normal salinity range may be less susceptible to heavy metal pollution than those living in salinities near the lower limit of their salinity tolerance (McLusky et al., 1986).

Corophium volutator is highly intolerant of metal pollution at levels often found in estuaries from industrial outfalls and contaminated sewage. A concentration 38 mg Cu/l was needed to kill 50% of Corophium volutator in 96 hour exposures (Bat et al., 1998). Other metals are far more toxic to Corophium volutator, e.g. zinc is toxic over 1 mg/l and toxicity to metals increases with increasing temperature and salinity (Bryant et al., 1985b). Mortality of 50% is caused by 14 mg/l (Bat et al., 1998). Although exposure to zinc may not be lethal, it may affect the perpetuation of a population by reducing growth and reproductive fitness. Mercury was found to be very toxic to Corophium volutator, e.g. concentrations as low as 0.1 mg/l caused 50% mortality in 12 days. Other metals known to be toxic include cadmium which causes 50% mortality at 12 mg/l (Bat et al., 1998), and arsenic, nickel and chromium which are all toxic over 2 mg/l (Bryant et al., 1984; Bryant et al., 1985a, 1985b).

Hydrocarbon & PAH contamination

Benchmark. Exposure of marine species or habitat to one or more relevant contaminants via uncontrolled releases or incidental spills. Further detail

Evidence

This pressure is Not assessed but evidence is presented where available.

Contamination at levels greater than the pressure benchmark may adversely impact the biotope. Light fractions (C10 - C19) of oils are much more toxic to Corophium volutator than heavier fractions (C19 - C40). In exposures of up to 14 days, light fraction concentrations of 0.1 g/kg sediment caused high mortality. It took 9 g/kg sediment to achieve similar mortalities with the heavy fraction (Brils et al., 2002). In the Forth Estuary, Corophium volutator was excluded for several hundred metres around the outfalls from hydrocarbon processing plants. Roddie et al. (1994) found high levels of mortality of Corophium at sites contaminated with crude oil.

Synthetic compound contamination

Benchmark. Exposure of marine species or habitat to one or more relevant contaminants via uncontrolled releases or incidental spills. Further detail

Evidence

This pressure is Not assessed but evidence is presented where available.

In general, crustaceans are widely reported to be intolerant of synthetic chemicals (Cole et al., 1999) and intolerance to some specific chemicals has been observed in amphipods. Species of a different genus are likely to differ in their susceptibility to synthetic chemicals and that this may be related to differences in their physiology (Powell, 1979).

Corophium volutator is paralysed by pyrethrum based insecticide sprayed onto the surface of the mud (Gerdol & Hughes, 1993) and pyrethrum would probably cause significant mortalities if it found its way into estuaries from agricultural runoff. Nonylphenol is an anthropogenic pollutant that regularly occurs in water bodies, it is an oestrogen mimic that is produced during the sewage treatment of non-ionic surfactants and can affect Corophium volutator (Brown et al., 1999). Nonylphenol is a hydrophobic molecule and often becomes attached to sediment in water bodies. This will make nonylphenol available for ingestion by Corophium volutator in estuaries where much of the riverine water-borne sediment flocculates and precipitates out of suspension to form mudflats. Nonylphenol is not lethal to Corophium volutator but does reduce growth and has the effect of causing the secondary antennae of males to become enlarged which can make the amphipods more vulnerable to predators (Brown et al., 1999). Corophium volutator is killed by 1% ethanol if exposed for 24 hours or more but can withstand higher concentrations in short pulses. Such short pulses, however, have the effect of rephasing the diel rhythm and will delay the timing of swimming activity for the duration of the ethanol pulse (Harris & Morgan, 1984b).

Radionuclide contamination

Benchmark. An increase in 10µGy/h above background levels. Further detail

Evidence

No evidence was found to support an assessment of biotope sensitivity. Corophium volutator readily absorbs radionuclides such as americium and plutonium from water and contaminated sediments (Miramand et al., 1982). However, the effect of contamination of the individuals was not known but accumulation through the food chain was assumed (Miramand et al., 1982).

Introduction of other substances

Benchmark. Exposure of marine species or habitat to one or more relevant contaminants via uncontrolled releases or incidental spills. Further detail

Evidence

This pressure is Not assessed.

De-oxygenation

Benchmark. Exposure to dissolved oxygen concentration of less than or equal to 2 mg/l for one week (a change from WFD poor status to bad status). Further detail

Evidence

Brafield (1964) concluded that the most significant factor influencing the oxygenation is the drainage of the beach which, in turn, is determined by the slope and particle size. Oxygen depletion becomes a severe problem at all states of the tide on only the very finest grained beaches, and as a general rule, if the percentage of particles of less than 0.25 mm median diameter exceeds 10% of a sediment, then the oxygen concentration of its interstitial water will be less than 20% of the air saturation level, and will drop rapidly during low tide periods (Brafield, 1964). Fine sands tend to have lower oxygen levels because their lower permeability leads to the trapping of detritus which, together with the large surface area for microbial colonization, leads to higher oxygen uptake (Eagle, 1983).

Laboratory studies by Khayrallah (1977) on Bathyporeia pilosa, indicated that it has a relatively poor resistance to conditions of hypoxia in comparison to other interstitial animals. However, Mettam (1989) and Sandberg (1997) suggest that Bathyporeia pilosa can survive short-term hypoxia. In a series of experiments Gamenick et al. (1996) suggested that Corophium volutator is highly sensitive to hypoxia and suffers 50% mortality after just 4 hours in hypoxic conditions, or in 2 hours if there is rapid build-up of sulphide (Gamenick et al., 1996). These results are largely in concordance with other work by Gamble (1970) who found that survival reates were temperature dependent with individuals surviving longer at lower temperatures. The level of oxygen was not assessed by Gamenick et al. (1996) and the description of the experimental set-up suggests that anoxic test conditions were used rather than hypoxic. Gamble (1970) found that at 5°C most individuals were inactive after 30 minutes exposure to anaerobic seawater and that mortality occurred later, the inactivity may have allowed the species to survive longer (Gamble, 1070). At 10°C, Corophium volutator survived for 22 h while Corophium arenarium survived 25 h.

Sensitivity assessment.  Exposure to air and the mixing effect of tidal currents will limit the intensity and duration of exposure to deoxygenated waters. The species characterizing the biotope are also mobile and able to migrate vertically or shorewards to escape unsuitable conditions. Biotope resistance is therefore assessed as ‘High’ and resilience as ‘High’ (by default), so that the biotope is considered to be ‘Not sensitive’.

Nutrient enrichment

Benchmark. Compliance with WFD criteria for good status. Further detail

Evidence

In-situ primary production is limited to microphytobenthos within and on sediments and the high levels of sediment mobility due to resuspension in strong tidal streams may limit the level of primary production as abrasion would be likely to damage diatoms (Delgado et al., 1991).

Sensitivity assessment.  In general primary production is low and this biotope is species poor. Nutrient level is not a key factor structuring the biotope at the pressure benchmark. Biotope resistance is therefore assessed as ‘High’, resilience as ‘High’ (by default), and the biotope is considered to be ‘Not sensitive’. Changes in nutrient status may indirectly affect this biotope where these result in changes in diatom production and inputs of macroalagal debris.

Organic enrichment

Benchmark. A deposit of 100 gC/m²/yr. Further detail

Evidence

The biotope description for the biotope LS.LSa.MoSa.AmSco  (JNCC, 2015) notes that inputs of organic matter seeping from the driftline can result in high abundances of Bathyporeia pilosa. Organic matter is unlikely to accumulate as sediment mobility and re-suspension by tidal currents  will result in removal of fine organic matter deposits.  

An increase in organic enrichment that exceeded the pressure benchmark may impact the habitat and biological assemblage, particularly in more sheltered areas where deposits can accumulate. For instance, prior to the introduction of a sewage treatment scheme in the Firth of Forth (Scotland), the communities of several sandy beaches were considerably modified by gross sewage pollution (Read et al., 1983).

Sensitivity assessment.  At the pressure benchmark organic inputs are likely to represent a food subsidy for the characterizing species and are unlikely to significantly affect the structure of the biological assemblage or impact the physical habitat. Biotope sensitivity is therefore assessed as ‘High’ and resilience as ‘High’ (by default), and the biotope is therefore considered to be ‘Not sensitive’.

Physical loss (to land or freshwater habitat)

Benchmark. A permanent loss of existing saline habitat within the site. Further detail

Evidence

All marine habitats and benthic species are considered to have a resistance of ‘None’ to this pressure and to be unable to recover from a permanent loss of habitat (Resilience is ‘Very low’). Sensitivity within the direct spatial footprint of this pressure is therefore ‘High’. Although no specific evidence is described, confidence in this assessment is ‘High’ due to the incontrovertible nature of this pressure

Physical change (to another seabed type)

Benchmark. Permanent change from sedimentary or soft rock substrata to hard rock or artificial substrata or vice-versa. Further detail

Evidence

The biotope is characterized by the sedimentary habitat (JNCC, 2015), a change to an artificial or rock substratum would alter the character of the biotope leading to reclassification and the loss of the sedimentary habitat and the characterizing amphipods and associated species such as the Baltic tellin, Macoma balthica.

Sensitivity assessment. Based on the loss of the biotope, resistance is assessed as ‘None’, recovery is assessed as ‘Very low’ (as the change at the pressure benchmark is permanent and sensitivity is assessed as ‘High’.

Physical change (to another sediment type)

Benchmark. Permanent change in one Folk class (based on UK SeaMap simplified classification). Further detail

Evidence

The pressure benchmark refers to the simplified Folk classification developed by Long (2006) and the UK Marine Habitat Classification Littoral and Sublittoral Sediment Matrices (Connor et al., 2004). The biotope is associated with medium to fine sands and muddy sand, a change at the pressure benchmark, therefore, refers to a change to muds and sandy muds or coarse sediments (gravelly sands and sandy gravels and gravels) and mixed sediments. The species present have specific sediment preferences related to burrowing or feeding. Experiments by Van Tomme et al. (2013) have shown that the optimal sedimentary habitats for Bathyporeia pilosa were fine sands, at a subtidal dredge disposal site the change to a finer muddy sediment led to a reduction in the abundance of Bathyporeia pilosa (Witt et al., 2004). Habitat partitioning has been observed between Corophium volutator and Corophium arenarium on the basis of sediments, although modified by disturbance from Arenicola marina and Cerastoderma edule (see removal of target species). Where the two Corophium species occur together Corophium arenarium is associated with areas of sandy sediment whilst Corophium volutator prefers muddier sediments (Watkin, 1941; Flach, 1993).

Sensitivity assessment. A change in sediment character would alter the character of the biotope which would be re-classified as another biotope type. A change in sediment type to a coarser, more mobile sediment such as coarse sands would favour Bathyporeia pilosa and other species present in mobile coarse sediments such as oligochaetes or the polychaetes Scolelepis squamata and the biotope would be reclassified within the LS.LSa.MoSa group. The presence of gravels could lead to the development of LS.LCS.Sh biotopes. Alternatively, where changes in hydrodynamic factors lead to the establishment of muddy sediment biotopes Corophium volutator already present in this biotope may be favoured while the habitat would be unsuitable for Corophium arenarium and Bathyporeia pilosa. The biotope present may revert to a more species rich, polychaete and bivalve dominated, shore depending on local conditions and larval transport.  Based on the loss of the biotope, resistance is assessed as ‘None’, recovery is assessed as ‘Very low’ (as the change at the pressure benchmark is permanent and sensitivity is assessed as ‘High’.

Habitat structure changes - removal of substratum (extraction)

Benchmark. The extraction of substratum to 30 cm (where substratum includes sediments and soft rock but excludes hard bedrock). Further detail

Evidence

Removal of the substratum to 30 cm would result in the loss of the characterizing species Corophium volutator is characteristic of muddy substrata while Corophium arenarium prefers sand; both construct U-shaped burrows in the surface 10 cm of the sediment (Meadows, 1964). Bathyporeia pilosa burrows between 0-10 cm in sediments (Nicolaisen & Kanneworff, 1969)

Sensitivity assessment. Removal of the sediment to 30 cm would remove the key characterizing species and their habitat. Resistance is therefore assessed as ‘None’. Resilience is assessed as ‘High’ if adjacent populations are present to support recolonization through adult migration and reproduction, so that sensitivity is assessed as ‘Medium’. However, if populations were removed over a wide area recovery could be prolonged and is assessed as ‘Medium’, so that sensitivity is assessed as ‘Medium’. The less precautionary assessment is considered more representative but the caveats outlined in the resilience section should be considered and this assessment may underestimate sensitivity.

Abrasion / disturbance of the surface of the substratum or seabed

Benchmark. Damage to surface features (e.g. species and physical structures within the habitat). Further detail

Evidence

The burrowing life habits of Corophium volutator are likely to provide some protection from abrasion at the surface only. However, any abrasion or physical disturbance is likely to reduce the density of Corophium spp. by emigration and increased mortality. For example, the sediment turnover caused by cockles and lugworms disturbs the burrows of Corophium volutator and caused a significant negative effect on Corophium volutator density as a result of increased rate of swimming making the amphipod more vulnerable to predation (Flach & De Bruin, 1993, 1994).  Corophium arenarium is also sensitive to sediment disturbance from bioturbating species (Flach, 1993).

A number of studies have assessed the effects of trampling on other intertidal amphipods and these assessments are used as a proxy. Comparisons between shores with low and high levels of trampling found that the amphipod Bathyporeia pelagica is sensitive to human trampling, other species including Pontocrates arenarius and the isopod Eurydice affinis also decreased in response to trampling but Bathyporeia pelagica appeared to be the most sensitive  (Reyes-Martínez et al., 2015). Changes in abundance of talitrid amphipods on urban beaches subject to high levels of recreational use was also observed by Bessa et al. (2014), this study compared abundances between samples taken ten years apart and thus the trends observed were not directly attributable to trampling vs beach cleaning or other pressures although they illustrate a general trend in density patterns as recreational use increases. Ugolini et al. (2008) carried out a controlled trampling experiment on Talitrus saltator. Plastic cylinders of 110 cm diameter (area 0.95 m²) were placed in the sand and all individuals trapped and counted, and 400 steps were made in a cylinder in 15 minutes after the amphipods had reburied. The trampling rate was based on observed number of beach users and therefore represents a realistic level of exposure. Alive individuals were counted at the end of the experiment and 24 hours after. Trampling significantly reduced abundance of the amphipods and after 24 hours the percentage of surviving amphipods dropped to almost zero, while survival rates of control (untrampled) amphipods were unaffected. Abrasion and compaction can therefore kill buried amphipods within sediments.

Sensitivity assessment. The trampling experiment (Ugolini et al., 2008) represents a high intensity of abrasion with multiple steps on the sediment within a short time period. The experiment does, however, demonstrate that amphipods are sensitive to abrasion and compaction of the sediment and these results are observed by comparisons between heavily and lightly used areas (Reyes-Martínez et al., 2015; Bessa et al., 2014). Corophium spp. are also sensitive to abrasion and sediment disturbance. Resistance to a single abrasion event is therefore assessed as ‘Low’ based on the characterizing species. Resilience is assessed as ‘High’, based on migration from adjacent populations and in-situ reproduction by surviving amphipods. Sensitivity is therefore assessed as ‘Low’. This assessment may underestimate sensitivity to high-levels of abrasion (repeated events within a short period). The trampling evidence and the evidence for penetration from mobile gears (see below) differ in the severity (resistance) of impact. This may be due to different levels of intensity (multiple trampling/abrasion events vs single penetration/towed gear impacts) or the nature of the pressure. Abrasion from trampling also involves a level of compaction that could collapse burrows and damage species through compression. Penetration may, however, break sediments open allowing mobile species to escape or species may be pushed forwards from towed gear by a pressure wave where this is deployed subtidally (Gilkinson et al., 1998). This suggests that exposure at different states of tide will mediate impacts. Both risk assessments are considered applicable to single events based on the evidence and the sensitivity assessment for both pressures is the same although resistance differs. 

Penetration or disturbance of the substratum subsurface

Benchmark. Damage to sub-surface features (e.g. species and physical structures within the habitat). Further detail

Evidence

In the Columbia river, no significant difference was found in Corophium volutator densities before and after dredging a channel and no difference between the dredged site and a control site (McCabe et al., 1998). Presumably, the dredging did cause mortality of Corophium volutator but recolonization was so rapid that no difference was found. The extraction of cockles by sediment raking and mechanical disturbance and digging for lugworms for bait is likely to cause significant mortality of Corophium volutator. Bait digging was found to reduce Corophium volutator densities by 39%, juveniles were most affected suffering a 55% reduction in dug areas (Shepherd & Boates, 1999).

Bergman and Santbrink (2000) found that direct mortality of gammarid amphipods, following a single passage of a beam trawl (in silty sediments where penetration is greater) was 28%. Similar results were reported from experiments s in shallow, wave disturbed areas, using a toothed, clam dredge. Bathyporeia spp. experienced a reduction of 25% abundance in samples immediately after intense clam dredging, abundance recovered after 1 day (Constantino et al., 2009). Experimental hydraulic dredging for razor clams resulted in  no statistically significant differences in Bathyporeia elegans abundances between treatments after 1 or 40 days (Hall et al., 1990), suggesting that recovery from effects was very rapid. Ferns et al. (2000) examined the effects of a tractor-towed cockle harvester on benthic invertebrates and predators in intertidal plots of muddy and clean sand. Harvesting resulted in the loss of a significant proportion of the most common invertebrates from both areas. In the muddy sand, the population of Bathyporeia pilosa remained significantly depleted for more than 50 days, whilst the population in clean sand recovered more quickly. These results agree with other experimental studies that clean sands tend to recover more quickly that other habitat types with higher proportions of fine sediment (Dernie et al., 2003).

Sensitivity assessment. Based on the evidence above it is considered that Bathyporeia spp. may be more resistant but Corophium species are likely to have ‘Low’ resistance to this pressure and resistance of the biotope is therefore assessed as ‘Low’. Recovery is assessed as ‘High’ and sensitivity is therefore categorised as ‘Low’.

Changes in suspended solids (water clarity)

Benchmark. A change in one rank on the WFD (Water Framework Directive) scale e.g. from clear to intermediate for one year. Further detail

Evidence

The characterizing species live within the sand and are unlikely to be directly affected by an increased concentration of suspended matter in the water column. Within the mobile sands habitat storm events or spring tides may re-suspend or transport large amounts of material and therefore species are considered to be adapted to varying levels of suspended solids.

Bathyporeia spp. feed on diatoms within the sand grains (Nicolaisen & Kanneworff, 1969), an increase in suspended solids that reduced light penetration could alter food supply. However, diatoms are able to photosynthesize while the tide is out and therefore a reduction in light during tidal inundation may not affect this food source, depending on the timing of the tidal cycle. Bathyporeia spp. may be regular swimmers within the surf plankton, where the concentration of suspended particles would be expected to be higher (Fincham, 1970a).

Sensitivity assessment. Increased organic solids in suspension may provide food to the key characterizing species. Increased inorganic suspended solids may increase abrasion but it is likely that the infaunal species would be unaffected. The biotope is considered to be ‘Not sensitive’ to a decrease in suspended solids that does not affect sediment transport and supply to the biotope. Biotope resistance is assessed as ‘Medium’ as some effects on feeding and diatom productivity may occur from increases in suspended solids, resilience is assessed as ‘High’, following a return to usual conditions and sensitivity is assessed as ‘Low’. This more precautionary assessment is presented in the table. Indirect effects such as deposition, erosion and associated sediment change that may result from changes in suspended solids in the long-term are assessed separately. 

Smothering and siltation rate changes (light)

Benchmark. ‘Light’ deposition of up to 5 cm of fine material added to the seabed in a single discrete event. Further detail

Evidence

The pressure benchmark for light deposition refers to the addition of a relatively thin layer of deposits in a single event. Species adapted to coarse sediments may not be able to burrow through fine sediments, or experienced reduced burrowing ability. For example, Bijkerk (1988, results cited from Essink, 1999) found that the maximal overburden through which Bathyporeia could migrate was approximately 20 cm in mud and 40 cm in sand. No further information was available on the rates of survivorship or the time taken to reach the surface.

Corophium volutator was categorized in AMBI sedimentation Group III – ‘species insensitive to higher amounts of sedimentation, but don’t easily recover from strong fluctuations in sedimentation’ (Gittenberger & Van Loon, 2011). Experimental fences placed on mudflats caused sedimentation rates of 2-2.5 cm/month and reduced Corophium volutator densities from approximately 1700 m² to approximately 400 m². In areas without fences, Corophium volutator numbers increased from approximately 1700 per m² to 3500 per m² (Turk & Risk, 1981).

Sensitivity assessment.  As the biotope is associated with shores exposed to strong tidal streams (JNCC, 2015), some sediment removal will occur, mitigating the effect of deposition. Bathyporeia pilosa is likely to be able to burrow through a 5 cm layer of fine sediments. Burrowing ability of Corophium spp. was unclear. Biotope resistance was therefore assessed as a precaution as ‘Medium’ as a deposit of 5 cm could negatively affect Corophium spp. Resilience was assessed as ‘High’ (by default). Biotope sensitivity is therefore assessed as ‘Low’ to this pressure. Repeated deposits or deposits over a large area or in sheltered systems that were shifted by wave and tidal action may result in sediment change (see physical change pressure).

Smothering and siltation rate changes (heavy)

Benchmark. ‘Heavy’ deposition of up to 30 cm of fine material added to the seabed in a single discrete event. Further detail

Evidence

Studies have found that beach ‘replenishment’ or ‘nourishment’ that involves the addition of sediments on beaches can have a number of impacts on the infauna (Peterson et al., 2000; Peterson et al., 2006). Impacts are more severe when the sediment added differs significantly in grain size or organic content (Nelson et al., 1989; Peterson et al., 2000). For example, Maurer et al. (1981) found that the amphipod Parahaustorius longimerus which occurs intertidally in clean, well-sorted sands and is an active, effective burrower was able to regain the surface after being buried by sand far more easily than when buried under silt/clay mixtures.

Little empirical information was found for the ability of characterizing species to reach the surface after burial. Bijkerk (1988, results cited from Essink, 1999) found that the maximal overburden through which Bathyporeia could migrate was approximately 20 cm in mud and 40 cm in sand. No further information was available on the rates of survivorship or the time taken to reach the surface and no information was available for other characterizing species. Corophium volutator was categorized in AMBI sedimentation Group III – ‘species insensitive to higher amounts of sedimentation, but don’t easily recover from strong fluctuations in sedimentation’ (Gittenberger & Van Loon, 2011). Experimental fences placed on mudflats caused sedimentation rates of 2-2.5 cm/month and reduced Corophium volutator densities from approximately 1700 m² to approximately 400 m². In areas without fences, Corophium volutator numbers increased from approximately 1700 per m² to 3500 per m² (Turk & Risk, 1981).

Repeated events are not considered at the pressure benchmark but it is noted that annual beach nourishment can alter beach sediments (see physical change pressure) and result in suppression of macroinvertebrate populations (Manning et al., 2014).

Sensitivity assessment. Sediment removal by wave action could mitigate the level of effect but overall smothering by fine sediments is likely to result in mortality of characterizing amphipods. Biotope resistance is therefore assessed as ‘Low’, resilience as High’, and biotope sensitivity is assessed as ‘Low’.

Litter

Benchmark. The introduction of man-made objects able to cause physical harm (surface, water column, seafloor or strandline). Further detail

Evidence

Not assessed. Corophium volutator is widely used in ecotoxicological studies and know to uptake nanoplastics, but toxicity at the current environmental relevant concentrations has yet to be confirmed (Booth et al., 2015). However, Corophium volutator forms an important food source for several species of birds and mobile predators such as fish and crabs (Hughes, 1988; Jensen & Kristensen, 1990; Raffaelli et al., 1991; Flach & De Bruin, 1994; Brown et al., 1999), which is likely to result in transition of the particles up the marine food chain. Nevertheless, there was insufficient evidence on which to assess the sensitivity of this biotope to the introduction of litter.

Electromagnetic changes

Benchmark. A local electric field of 1 V/m or a local magnetic field of 10 µT. Further detail

Evidence

No evidence for the characterizing species was found to assess this pressure. For some amphipods there is evidence for geomagnetic orientation being inhibited or disrupted by the presence of electromagnetic fields or by changing magnetic fields. Arendse & Barendregt (1981) manipulated magnetic fields to alter orientation of the talitrid amphipod Orchestia cavimana. 

Deep-water amphipods Gondogenia arctica have been shown to be sensitive to even weak electromagnetic fields which cancel magnetic orientation (Tomanova & Vacha, 2016). Loss of orientation was observed at a radiofrequency electromagnetic field of 2 nT (0.002  µT) (Tomanova & Vacha, 2016).

Underwater noise changes

Benchmark. MSFD indicator levels (SEL or peak SPL) exceeded for 20% of days in a calendar year. Further detail

Evidence

Corophium spp. and Bathyporeia pilosa are probably sensitive to surface vibrations but little is known about the effects of noise on invertebrates. However, the species are unlikely to be affected by noise pollution and so the biotope is assessed as ‘Not sensitive’.

Introduction of light or shading

Benchmark. A change in incident light via anthropogenic means. Further detail

Evidence

As this feature is not characterized by the presence of primary producers it is not considered that shading would alter the character of the habitat. No specific evidence was found to assess sensitivity of the characterizing species to this pressure. Changes in light levels may, however, affect activity rhythms of the invertebrates. Bathyporeia spp. emerge at night and therefore an increase in light may inhibit activity, particularly at night when they emerge from the sediment and are most active (Jelassi et al., 2015; Ayari, 2015). Hartwick (1976), for example, found that artificial lighting interfered with learning or orientation cues by Talitrids. Corophium spp.  live in burrows and may not be affected by changes in light although males emerge and crawl over sediments (Meadows, 1964).

Orientation by light has been well studied for intertidal amphipods (particularly Talitrus saltator). Intertidal amphipods orientate themselves by a range of factors that include (but are not limited to) visual cues based on solar or astronomic cues such as the moon and the geomagnetic field (Scapini, 2014). Activity patterns are also linked to internal biological clocks that respond to diel, tidal, lunar and seasonal cycles, so that animals are active during the most suitable time of day or night (Scapini, 2014). The introduction of light or an increase in shading could therefore alter behavioural patterns and navigation. As responses may be species specific or vary according to local factors or individual needs such as feeding, mating, it is not possible to provide a simple assessment for this species. Some sensitivity is however likely if incident light levels were altered. This will depend, however, on the footprint and intensity of impact. Fanini et al. (2014) found no difference in abundance of Talitrus saltator between Greek beaches that frequently hosts small scale beach-parties with lights at night and those that were not used in this way. 

Changes in light and level of shade may indirectly affect the characterizing amphipods through changes in food supply via photosynthesis of diatoms within sediments. Benthic microalgae play a significant role in system productivity and trophic dynamics, as well as habitat characteristics such as sediment stability (Tait & Dipper, 1998). Shading could prevent photosynthesis leading to death or migration of sediment diatoms altering sediment cohesion and food supply to the characterizing amphipods.

Sensitivity assessment. Changes in light are not considered to directly affect the biotope, however, some changes in the behaviour of Bathyporeia spp. and food supply for the characterizing amphipods could result. Biotope resistance is assessed as ‘Medium’ and resilience is assessed as ’High’. Biotope sensitivity is therefore assessed as ‘Low’.

Barrier to species movement

Benchmark. A permanent or temporary barrier to species movement over ≥50% of water body width or a 10% change in tidal excursion. Further detail

Evidence

As the amphipods that characterize this biotope have benthic dispersal strategies (via brooding), water transport is not a key method of dispersal over wide distances, as it is for some marine invertebrates that produce pelagic larvae. Where populations were removed changes in water transport of adults may, however, be reduced by changes in local hydrodynamics preventing recolonization. Conversely, a barrier may enhance local connectivity by reducing the loss of adults from the system.

Sensitivity assessment. The biotope (based on the brooding life strategy of the characterizing species) is considered to have ‘High’ resistance to the presence of barriers that lead to a reduction in tidal excursion, resilience is assessed as ‘High’ (by default) and the biotope is considered to be ‘Not sensitive’.

Death or injury by collision

Benchmark. Injury or mortality from collisions of biota with both static or moving structures due to 0.1% of tidal volume on an average tide, passing through an artificial structure. Further detail

Evidence

'Not relevant’ to seabed habitats. NB. Collision by grounding vessels is addressed under ‘surface abrasion'.

Visual disturbance

Benchmark. The daily duration of transient visual cues exceeds 10% of the period of site occupancy by the feature. Further detail

Evidence

In substratum choice experiments, Corophium volutator preferred substrata independently of whether the dishes were in darkness or illuminated (Meadows, 1964). Bathyporeia pilosa emerges from the sediments at night and are unlikely to be disturbed although like many species they may flee from movements. Therefore, visual disturbance is probably 'Not relevant'.

Genetic modification & translocation of indigenous species

Benchmark. Translocation of indigenous species or the introduction of genetically modified or genetically different populations of indigenous species that may result in changes in the genetic structure of local populations, hybridization, or change in community structure. Further detail

Evidence

Key characterizing species within this biotope are not cultivated or translocated. This pressure is therefore considered ‘Not relevant’ to this biotope group.

Introduction or spread of invasive non-indigenous species

Benchmark. The introduction of one or more invasive non-indigenous species (INIS). Further detail

Evidence

The American slipper limpet Crepidula fornicata was introduced to the UK and Europe in the 1870s from the Atlantic coasts of North America with imports of the eastern oyster Crassostrea virginica. It was recorded in Liverpool in 1870 and the Essex coast in 1887-1890. It has spread through expansion and introductions along the full extent of the English Channel and into the European mainland (Blanchard, 1997, 2009; Bohn et al., 2012, 2013a, 2013b, 2015; De Montaudouin et al., 2018; Helmer et al., 2019; Hinz et al., 2011; McNeill et al., 2010; Powell-Jennings & Calloway, 2018; Preston et al., 2020; Stiger-Pouvreau & Thouzeau, 2015).

Crepidula fornicata is recorded from shallow, sheltered bays, lagoons and estuaries or the sheltered sides of islands, in variable salinity (18 to 40) although it prefers ca 30 (Tillin et al., 2020). Larvae require hard substrata for settlement. It prefers muddy gravelly, shell-rich, substrata that include gravel, or shells of other Crepidula, or other species e.g., oysters, and mussels. It is highly gregarious and seeks out adult shells for settlement, forming characteristic ‘stacks’ of adults. But it also recorded in a wide variety of habitats including clean sands, artificial substrata, Sabellaria alveolata reefs and areas subject to moderately strong tidal streams (Blanchard, 1997, 2009; Bohn et al., 2012, 2013a, 2013b, 2015; De Montaudouin et al., 2018; Hinz et al., 2011; Powell-Jennings & Calloway, 2018; Preston et al., 2020; Stiger-Pouvreau & Thouzeau, 2015; Tillin et al., 2020).

High densities of Crepidula fornicata cause ecological impacts on sedimentary habitats. The species can form dense carpets that can smother the seabed in shallow bays, changing and modifying the habitat structure. At high densities, the species physically smothers the sediment, and the resultant build-up of silt, pseudofaeces, and faeces is deposited and trapped within the bed (Tillin et al., 2020, Fitzgerald, 2007, Blanchard, 2009, Stiger-Pouvreau & Thouzeau, 2015). The biodeposition rates of Crepidula are extremely high and once deposited, form an anoxic mud, making the environment suitable for other species, including most infauna (Stiger-Pouvreau & Thouzeau, 2015, Blanchard, 2009). For example, in fine sands, the community is replaced by a reef of slipper limpets, that provide hard substrata for sessile suspension-feeders (e.g., sea squirts, tube worms and fixed shellfish), while mobile carnivorous microfauna occupy species between or within shells, resulting in a homogeneous Crepidula dominated habitat (Blanchard, 2009). Blanchard (2009) suggested the transition occurred and became irreversible at 50% cover of the limpet. De Montaudouin et al. (2018) suggested that homogenization occurred above a threshold of 20-50 Crepidula /m².

Impacts on the structure of benthic communities will depend on the type of habitat that Crepidula colonizes. De Montaudouin & Sauriau (1999) reported that in muddy sediment dominated by deposit-feeders, species richness, abundance and biomass increased in the presence of high densities of Crepidula (ca 562 to 4772 ind./m²), in the Bay of Marennes-Oléron, presumably because the Crepidula bed provided hard substrata in an otherwise sedimentary habitat. In medium sands, Crepidula density was moderate (330-1300 ind./m²) but there was no significant difference between communities in the presence of Crepidula. Intertidal coarse sediment was less suitable for Crepidula with only moderate or low abundances (11 ind./m²) and its presence did not affect the abundance or diversity of macrofauna. However, there was a higher abundance of suspension–feeders and mobile Crustacea in the absence of Crepidula (De Montaudouin & Sauriau, 1999). The presence of Crepidula as an ecosystem engineer has created a range of new niche habitats, reducing biodiversity as it modifies habitats (Fitzgerald, 2007). De Montaudouin et al. (1999) concluded that Crepidula did not influence macroinvertebrate diversity or density significantly under experimental conditions, on fine sands in Arcachon Bay, France. De Montaudouin et al. (2018) noted that the limpet reef increased the species diversity in the bed, but homogenised diversity compared to areas where the limpets were absent. In the Milford Haven Waterway (MHW), the highest densities of Crepidula were found in areas of sediment with hard substrata, e.g., mixed fine sediment with shell or gravel or both (grain sizes 16-256 mm) but, while Crepidula density increased as gravel cover increased in the subtidal, the reverse was found in the intertidal (Bohn et al., 2015). Bohn et al. (2015) suggested that high densities of Crepidula in high-energy environments were possible in the subtidal but not the intertidal, suggesting the availability of this substratum type is beneficial for its establishment. Hinz et al. (2011) reported a substantial increase in the occurrence of Crepidula off the Isle of Wight, between 1958 and 2006, at a depth of ca 60 m, on hard substrata (gravel, cobbles, and boulders), swept by strong tidal streams. Presumably, Crepidula is more tolerant of tidal flow than the oscillatory flow caused by wave action which may be less suitable (Tillin et al., 2020).

The availability of hard substrata (e.g., gravel) may only restrict initial colonization as higher densities of Crepidula function as substrata for subsequent colonization (Thieltges et al., 2004; Blanchard, 2009). However, Bohn et al. (2015) noted that Crepidula occurred at low density or was absent in areas of homogenous fine sediment and areas dominated by boulders. Bohn et al. (2015) suggested that wave action (exposure) probably prevented the establishment of large numbers of Crepidula in high-energy areas. Blanchard (2009) noted that sandy areas in the Bay of Saint-Mont Michel were not colonized by Crepidula because of surface sand mobility. Thieltges et al. (2003) also noted that storm events removed some clumps of mussels and presumably Crepidula onto tidal flats where they disappeared, which caused their abundance to fluctuate. Similarly, Crepidula was absent from sandy substrata in Swansea Bay but was most abundant in the shelter of the breakwater at the Swansea east site (Powell-Jennings & Calloway, 2018). Powell-Jennings & Calloway (2018) noted that Crepidula is killed by sudden burial and possibly burial due to deposition, which could mitigate Crepidula density. In addition, in the MHW, the highest densities of Crepidula were found in areas of sediment with hard substrata, e.g., mixed fine sediment with shell, or gravel or both but, while Crepidula density increased as gravel cover increased in the subtidal, the reverse was found in the intertidal (Bohn et al., 2015). However, gravel formed the base of most stacks of Crepidula in the intertidal, which suggested that initial colonization occurred on available hard substrata (i.e., gravel) in the absence of adult shells of Crepidula (Bohn et al., 2015).

Crepidula is recorded from the lower intertidal to ca 160 m in depth but it is most common in the shallow subtidal and low water springs (Blanchard, 1997; Thieltges et al., 2003; Bohn et al., 2012, 2015; Hinz et al., 2011; OBIS, 2023; Tillin et al., 2020). Bohn et al. (2012, 2013a, 2013b, 2015) suggested that extreme conditions in intertidal limited its upward distribution due to early post-settlement mortality. It reached its highest densities on the lower shore (below ca 0.7 m) and was absent from the high tidal level (ca 1.8 m) in the MHW (Bohn et al., 2015). Bohn et al. (2013b) noted that Crepidula spat in their experimental intertidal panels suffered high mortality of 78-100% during emersion by low water spring tides. Thieltges et al. (2003) noted that Crepidula abundance at the intertidal to the subtidal transition zone (ca 21/ m²) was significantly higher than in the upper, mid, and lower intertidal ca <3/ m²). Similarly, Diederich & Pechenik (2013) noted that Crepidula densities were not significantly different in the low intertidal (+0.2 m) and shallow subtidal (-1 m) but became lower at +0.4 and were absent above +0.6 m in Bissel Cove, Rhode Island where the mean high water was +1.38 m. They reported that intertidal adults experienced temperatures of ca 42°C, which were 15°C higher than subtidal adults. However, there was no significant difference in the tolerance of subtidal and intertidal adults with a lethal range of 33-37°C after three hours in the laboratory. Diederich & Pechenik (2013) suggested that adult Crepidula were living close to their upper thermal limit in Rhode Island and would be driven into the subtidal due to climate change. Diederich et al. (2015) reported that most juvenile Crepidula died after aerial exposure under laboratory conditions (20°C, 75% relative humidity), while adults from the intertidal and subtidal survived (26°C, 75% relative humidity). Franklin et al. (2023) noted that the body mass index of adult Crepidula did not decrease significantly in winter months in New Hampshire, USA, but did decrease in spring and summer, probably due to its investment in reproduction. 

The density of Crepidula populations in northern Europe (Germany, Denmark, and Norway) was significantly lower (ca <100/ m2) than in southern waters. Thieltges et al. (2004) reported that the population of Crepidula was affected strongly by cold winters in the Wadden Sea. The winters of 2001 and 2003 resulted in ca 56-64% mortality of intertidal Crepidula and up to 97% on one mussel bed, compared to only 11-14% in southern areas without frost. Crepidula almost vanished from the Wadden Sea after the 1978/79 winter and took ten years to recover due to moderate winters which regularly affected the population. Similarly, 25% mortality was observed in Crepidula populations on the south coast of the UK after the extreme 1962/63 winter (Crisp, 1964, Bohn et al., 2012). Thieltges et al. (2003) suggested that global warming may allow Crepidula populations to become more abundant in northern Europe.

The sand sediments characterizing this biotope are likely to be mobile due to strong tidal streams and contain little organic matter, and the biotope occurrence in variable salinity, limits the establishment of marine and coastal invasive non-indigenous species as the habitat conditions are unsuitable for most species, as exemplified by the low species richness characterizing this biotope. On the upper shore colonization of sandy mud flats by cord grass Spartina anglica may, however, affect the distribution of amphipods. In the Dovey Estuary, the spread of this species has restricted the distribution of Corophium spp. to creeks and drainage channels (Fish & Mills, 1979).

Sensitivity assessment. The above evidence suggests that this biotope is unsuitable for the colonization of Crepidula fornicata due to a lack of gravel, shells, or any other hard substrata used for larvae settlement (Tillin et al., 2020), especially in moderately exposed examples in which storms may mobilise the sediment (JNCC, 2022). Despite the extremely sheltered examples of the biotope that would otherwise be suitable for Crepidula, the mobility of the sediment is unsuitable and makes it unlikely for Crepidula to become established. There may be higher densities of Crepidula in the lower shore examples of the biotope, but the densities may be lower in the mid-shore, and it may be absent from the upper shore due to the unsuitable extreme conditions in the intertidal zone preventing Crepidula post-settlement recruitment and mitigating colonization (Bohn et al. 2015). Colonization of the upper shore by Spartina anglica may result in loss of the characterizing amphipods. However, this is likely to only affect upper shore examples. Therefore, in terms of Crepidula fornicata, resistance is assessed as 'High' and resilience as 'High' so the biotope is assessed as 'Not sensitive'. The confidence in the assessment is 'Low' because the sensitivity of this biotope to Crepidula is potentially site-specific, there is a risk of its introduction by artificial means, and the is a lack of direct evidence of Crepidula being reported to occur in the biotope. Further evidence is required. However, colonization of the upper shore by Spartina anglica may result in loss of the characterizing amphipods, but this is likely to only affect upper shore examples. Therefore, due to Spartina anglica colonization, resistance is assessed as 'Medium' and resilience as 'Very low' so the biotope is assessed as 'Medium'.

Introduction of microbial pathogens

Benchmark. The introduction of relevant microbial pathogens or metazoan disease vectors to an area where they are currently not present (e.g. Martelia refringens and Bonamia, Avian influenza virus, viral Haemorrhagic Septicaemia virus). Further detail

Evidence

Amphipods may be infected by a number of parasites or pathogens that alter population numbers through changes in host condition, growth, behaviour and reproduction (Green Extabe & Ford, 2014). Infection by acanthocephalan larvae, for example, may alter behaviour and responses of gammarid amphipods (Bethel & Holmes, 1977). The amphipod Orchestia gammarellus is host to the parasitic protist Marteilia which has a feminizing effect on populations, with higher ratios of females and intersex males in infected, estuarine populations (Ginsburger-Vogel & Desportes, 1979).

Corophium volutator is parasitized by several species of trematodes in Europe and North American (McCurdy et al., 2000a; McCurdy et al., 2000b; Mouritsen & Jensen, 1997, cited in Shim et al., 2013). Mass mortalities of Corophium volutator have been associated with infestation by trematodes in the Wadden Sea (Jensen & Mouritsen, 1992). A dense field of Corophium volutator disappeared completely, and the density of the mud snail Hydrobia ulvae declined by 40% during spring 1990 as a result of an epizootic by trematodes. High spring temperature accelerated both the development rate and the release of infective larval stages of an infectious trematode from the snail Hydrobia ulvae (Poulin & Mouritsen, 2006). Studies conducted in the Baltic Sea suggested that increased parasitism by trematode species has a detrimental effect on local amphipods (Meissner & Bick, 1999; Mouritsen & Jensen, 1997 cited in Shim et al., 2013).  

Sensitivity assessment. Although no records of the biotope being affected by the introduction of microbial pathogens in the British Isles, mass mortality of characterizing species Corophium volutator has been observed (Jensen & Mouritsen, 1992). The biotope character is therefore considered to be at some risk and resistance is judged to be ‘Low’ as Bathyporeia spp. and other associated species such as Hydrobia ulvae may be affected increasing the overall impact of the biological assemblage. Resilience is assessed as ‘High’ (where the disease-causing organisms decline) and the biotope is assessed as having ‘Low’ sensitivity to the introduction of microbial pathogens. Sensitivity will be higher where pathogens persist, preventing recovery.

Removal of target species

Benchmark. Removal of species targeted by fishery, shellfishery or harvesting at a commercial or recreational scale. Further detail

Evidence

The characterizing species are not directly targeted and therefore this pressure is not considered relevant. Some polychaetes and bivalves that characterize intertidal sand flats and muddy sand habitats such as Arenicola marina and Cerastoderma edule may reduce abundance of Corophium volutator and Corophium arenarium (Flach, 1993). Removal of these species through recreational and commercial harvesting may therefore increase the suitability of some areas for the characterizing species. However, this effect is not assessed for this biotope. 

Removal of non-target species

Benchmark. Removal of features or incidental non-targeted catch (by-catch) through targeted fishery, shellfishery or harvesting at a commercial or recreational scale. Further detail

Evidence

Direct, physical impacts are assessed through the abrasion and penetration of the seabed pressures, while this pressure considers the ecological or biological effects of by-catch. The characterizing species in this biotope are highly likely to be damaged or directly removed by static or mobile gears that are targeting other species (see abrasion and penetration pressures).

The loss of the key characterizing species through unintentional removal would alter the character of the biotope and may lead to reclassification to a similar, species-poor biotope such as LS.LSa.MoSa.BarSa. The ecosystem services such as secondary production and food for higher trophic levels such as birds provided by the amphipods would be lost.  A dense field of Corophium volutator in the Wadden Sea disappeared completely during spring 1990 as a result of an epizootic by trematodes. The collapse of its population led to drastic changes in erosion patterns, sediment characteristics, and microtopography, as well as marked changes in the abundance of other macrofaunal species in the mudflat (Poulin & Mouritsen, 2006).

Sensitivity assessment. Biotope resistance to loss of the characterizing species is assessed as ‘Low’ as the burrowing lifestyle and mobility of characterizing species mean that a proportion of the population may escape incidental removal. Resilience is assessed as ‘High’ based on in-situ recovery and migration from adjacent populations and sensitivity is therefore assessed as ‘Low’.